Journal: Biomedicines

Article Title: Integrated Multi-Omics Analysis Reveals Immune and Metabolic Dysregulation in a Restraint Stress-Induced Depression Model

doi: 10.3390/biomedicines13092183

Figure Lengend Snippet: Transcriptomic profiling. ( a ) Diagram of the 24-hour restraint stress mouse model. ( b ) Quantification of the sucrose preference in SPT (** p = 0.007) and immobility time in 4 min in FST (* p = 0.0121, n = 17 for NC group and n = 16 for restraint group, each dot represents one mouse). ( c ) Volcano plot for differentially expressed genes in restraint mice samples compared with controls in RNA-seq. Blue and red dots indicate statistical down- and up-regulated genes, respectively. ( d ) Heat-map analysis on expressions of genes in the mPFC ( n = 3 for each group). ( e , f ) GO—‘Biological Processes’/‘Cellular Component’/‘Molecular Function’ analysis on up-regulated genes in restraint mice compared with control mice in RNA-seq. ( g , h ) GSEA analysis of differentially expressed genes in restraint mice samples compared with controls in RNA-seq. Red indicates gene sets positively enriched in the stress group (NES > 0), while blue indicates gene sets negatively enriched in stress (NES < 0). NES, normalized enrichment score.

Article Snippet: The immobility time of the last 4 min were analyzed by Noldus-maze.

Techniques: RNA Sequencing, Control

Journal: Biomedicines

Article Title: Integrated Multi-Omics Analysis Reveals Immune and Metabolic Dysregulation in a Restraint Stress-Induced Depression Model

doi: 10.3390/biomedicines13092183

Figure Lengend Snippet: Clemastine treatment alleviates depressive-like behavior. ( a , b ) Behavioral assessments show that clemastine treatment restores sucrose preference (( a ), Control + Water versus Restraint + Water, * p = 0.0155; Restraint + Water versus Restraint + Clemastine, * p = 0.0439; n = 10 for Control group and n = 12 for restraint group) and reduces immobility time (( b ), Control + Water versus Restraint + Water, * p = 0.0297; Restraint + Water versus Restraint + Clemastine, * p = 0.0452; n = 10 for Control group and n = 12 for restraint group) in restraint-stressed mice, indicating antidepressant-like effects. Each dot represents one mouse. ( c ) Temporal clustering analysis of proteomics datasets of mPFC tissues from mice (N means control group, C means control + clemastine group, R means restraint group, R + C means restraint+clemastine group, n = 3 for each group).

Article Snippet: The immobility time of the last 4 min were analyzed by Noldus-maze.

Techniques: Control